Eppendorf 5402 Manual Site

Elias carefully closed the lid, but he didn't put the manual back in the drawer. He set it on the benchtop, right next to his pipettes.

Ensure tubes are filled to identical volumes. Density differences require balancing by mass, not just volume. eppendorf 5402 manual

The 5402 does not calculate relative centrifugal force (RCF) automatically. You need the manual’s rotor-specific radius values to manually convert rpm to g. For pelleting RNA or separating subcellular fractions, that formula is non-negotiable. Elias carefully closed the lid, but he didn't

Locate the small plastic plug or opening underneath or on the side of the unit (depending on the exact manufacturing year batch). Density differences require balancing by mass, not just

The Eppendorf 5402 features basic internal diagnostics. When an issue occurs, it often generates an error signal or specific operational symptoms. Symptom / Error Potential Cause Corrective Action Asymmetrical tube loading or broken tube inside the rotor.

Turn the temperature dial to the desired setting. Use the "Fast Cool" function to pre-cool the rotor chamber before spinning sensitive RNA or protein samples.

Provides digital readouts and controls for time, temperature, and speed. Short-Spin Function: Includes a momentary button